LOCUS       sequence-89601-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of METTL14 (methyltransferase-like 14) in human 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng 
            X, Dai Q, Chen W, He C
  TITLE     A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine
            methylation.
  JOURNAL   Nat Chem Biol. 2014 Feb;10(2):93-5. doi: 10.1038/nchembio.1432. Epub
            2013 Dec 6.
  PUBMED    24316715
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1432"; journalName: "Nat Chem Biol"; date: 
            "2014-02"; volume: "10"; issue: "2"; pages: "93-5"
COMMENT     Sequence Label: pcDNA3/Flag-METTL14
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccatggatta caaggatgac
      121 gatgacaagg atagccgctt gcaggagatc cgggagcggc agaagttacg gcgacagctc
      181 ctcgcgcagc agttgggagc tgaaagtgcc gacagcattg gtgccgtgtt aaatagcaaa
      241 gatgagcaga gagaaattgc tgaaacaaga gaaacttgca gggcttccta tgatacctct
      301 gctccaaatg caaaacgtaa gtatctggat gaaggagaga cagatgaaga caaaatggaa
      361 gaatataagg atgaactaga aatgcaacag gatgaagaaa atttgccata tgaagaagag
      421 atttacaaag attctagtac ttttcttaag ggaacacaga gcttaaatcc ccataatgat
      481 tactgccaac attttgtaga cactggacat agacctcaga atttcatcag ggatgtaggt
      541 ttagctgaca gatttgaaga atatcctaaa ctgagggagc tcatcaggct aaaggatgag
      601 ttaatagcta aatctaacac tcctcccatg tacttacaag ccgatataga agcctttgac
      661 atcagagaac taacacccaa atttgatgtg attcttctgg aacccccttt agaagaatat
      721 tacagagaaa ctggcatcac tgctaatgaa aaatgctgga cttgggatga tattatgaag
      781 ttagaaattg atgagattgc agcacctcga tcatttattt ttctctggtg tggt
//
