LOCUS       sequence-89608-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Karl Kramer plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BirA(R118G)-HA destination vector
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    118..342
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     331..348
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(379..405)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
       61 tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca
      121 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
      181 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
      241 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
      301 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggt
      361 ttaaacttag gccgcctatg cgtaatccgg tacatcgtaa gggtaagcct tctctgcgct
      421 tctcagggag atttctccgc ccatccaggg cttgatgatt ccgtcctgct ccagcagcag
      481 agctccctgc ttgtcgattc ctctggagat gccgaagatt tccttgtcgc cgatgatcag
      541 cttcacaggt ctgttgatga agttgtccag cttctcccat ctgctcaggt aaggagccag
      601 tccctcctgc tcgaacagct ccagagcggc tctcagctct ctgatcagca tggcggccag
      661 ggtgttccgg tccaggttga tgccagcctc ctgcagggtg atccagccct ggttcaccac
      721 gctctcctcc actcttctca tggccatgtt gattccggct ccgatcacga tctgagcggc
      781 atcgcctgtc ttgcctgtca gctccaccag gatgccagcc agcttgcggt cctggaggta
      841 caggtcatta ggccacttca ctctcacctt gtcggctccc agctttctca gcacctcggc
      901 catcacgatt ccgatcacca ga
//
