LOCUS       sequence-89611-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for PhiC31 integrase-mediated cassette exchange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT378_pattB-pCA-GFP-pA-attB
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..122
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        428..731
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacgat gtaggtcacg gtctcgaagc
       61 cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca
      121 tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc
      181 gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac
      241 gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca
      301 cggcgggcat gtcgacggta tcgataagct tcccttaatt aaccgtttaa acaattctgc
      361 aggaatctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      421 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      481 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      541 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      601 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      661 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      721 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc t
//
