LOCUS       sequence-89613-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Phic31 integrase-mediated transgene integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT316_pattB-pCA-GFP-pA
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..136
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        442..745
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga cgatgtaggt
       61 cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
      121 ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc
      181 ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac
      241 ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg
      301 gttctcgacg gtcacggcgg gcatgtcgac ggtatcgata agcttccctt aattaaccgt
      361 ttaaacaatt ctgcaggaat ctagttatta atagtaatca attacggggt cattagttca
      421 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      481 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      541 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      601 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      661 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      721 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      781 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      841 agcgatgg
//
