LOCUS       sequence-89625-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains human Histone H2B fused to EGFP in the pCS2+ expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Megason SG
  TITLE     In toto imaging of embryogenesis with confocal time-lapse 
            microscopy.
  JOURNAL   Methods Mol Biol. 2009;546:317-32. doi: 
            10.1007/978-1-60327-977-2_19.
  PUBMED    19378112
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "546:317-32. doi"; pages: " 
            10.1007/978-1-60327-977-2_19"
COMMENT     Sequence Label: pCS-H2B-EGFP
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             186..563
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      576..585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(582..584,585..587,588..1046)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(627..648)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(888..907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt
      121 cgaattcaag gcctctcgag cctctagaac tatagtgagt cgtattactc gacggtaccg
      181 ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
      241 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
      301 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
      361 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
      421 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
      481 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
      541 tcaccaagta caccagcgct aaggatccac cggtcgccac catggtgagc aagggcgagg
      601 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      661 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      721 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      781 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      841 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      901 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      961 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1021 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1081 agatccgc
//
