LOCUS       sequence-89627-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to monomeric red fluorescent 
            protein 1 in the pCS2+ expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Megason SG
  TITLE     In toto imaging of embryogenesis with confocal time-lapse 
            microscopy.
  JOURNAL   Methods Mol Biol. 2009;546:317-32. doi: 
            10.1007/978-1-60327-977-2_19.
  PUBMED    19378112
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "546:317-32. doi"; pages: " 
            10.1007/978-1-60327-977-2_19"
COMMENT     Sequence Label: pCS-H2B-mRFP1
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             186..563
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      576..585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(712..730)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     980..999
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt
      121 cgaattcaag gcctctcgag cctctagaac tatagtgagt cgtattactc gacggtaccg
      181 ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
      241 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
      301 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
      361 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
      421 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
      481 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
      541 tcaccaagta caccagcgct aaggatccac cggtcgccac catggcctcc tccgaggacg
      601 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
      661 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      721 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct cagttccagt
      781 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      841 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      901 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      961 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     1021 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagatg aggctgaagc
     1081 tgaagg
//
