LOCUS       sequence-89628-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to monomeric red fluorescent 
            protein 1 in the pCS2+ expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Megason SG
  TITLE     In toto imaging of embryogenesis with confocal time-lapse 
            microscopy.
  JOURNAL   Methods Mol Biol. 2009;546:317-32. doi: 
            10.1007/978-1-60327-977-2_19.
  PUBMED    19378112
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "546:317-32. doi"; pages: " 
            10.1007/978-1-60327-977-2_19"
COMMENT     Sequence Label: pCS-H2B-mRFP1
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    156..290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(297..974)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(297..974)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(359..382)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(557..576)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     826..844
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      971..980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac
       61 cgggcccaat gcattggcgc cgcggccgcg aattaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tacgtattag
      301 gcgccggtgg agtggcggcc ctcggcgcgc tcgtactgtt ccacgatggt gtagtcctcg
      361 ttgtgggagg tgatgtccag cttgatgtcg gtcttgtagg cgccgggcag ctgcacgggc
      421 ttcttggcca tgtaggtggt cttgacctcg gcgtcgtagt ggccgccgtc cttcagcttc
      481 agcctcatct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggtggag
      541 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
      601 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
      661 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      721 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtactgg
      781 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc
      841 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
      901 tggccgttca cggagccctc catgcgcacc ttgaagcgca tgaactcctt gatgacgtcc
      961 tcggaggagg ccatggtggc gaccggtgga tccttagcgc tggtgtactt ggtgatggcc
     1021 ttagtaccct cggacacggc gtgcttggcc aactccccag gcagcagcag gcgcacggcc
     1081 gtctggatct ccctggaggt gatggtcgag cgcttgttgt aatgcgccag gcg
//
