LOCUS       sequence-89648-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of 3xHA tagged human TERT, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pCDNA-3xHA-hTERT
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             226..252
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc ttggtaccga gctcggatcc actagtccag
      121 tgtggtggaa ttctgcagat aaacgccacc atggggcaac aaggataccc gtatgatgtt
      181 ccggattacg ctggctaccc atacgacgtc ccagactacg ctggctaccc atacgacgtc
      241 ccagactacg ctcaaggagt gcggccgcgg gggcgcgccc cgcgcgctcc ccgctgccga
      301 gccgtgcgct ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg
      361 cggcgcctgg ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc
      421 gcgctggtgg cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc
      481 ccctccttcc gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg
      541 tgcgagcgcg gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc
      601 gggggccccc ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc
      661 gacgcactgc gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg
      721 ctggttcacc tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac
      781 caggtgtgcg ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca
      841 cacg
//
