LOCUS       sequence-89665-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  904 bp insert from human INS gene used as a control for 
            methylation-specific PCR assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K
  TITLE     Development of a quantitative methylation-specific polymerase chain 
            reaction method for monitoring beta cell death in type 1 diabetes.
  JOURNAL   PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub
            2012 Oct 29.
  PUBMED    23144715
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47942. doi"; pages: " 
            10.1371/journal.pone.0047942. Epub 2012 Oct 29"
COMMENT     Sequence Label: pCR2.1-INS-904
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     708..779
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
ORIGIN
        1 cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga
       61 tggatatctg cagaattcgc cctttgtggg gacaggggtc tggggacagc agcgcaaaga
      121 gccccgccct gcagcctcca gctctcctgg tctaatgtgg aaagtggccc aggtgagggc
      181 tttgctctcc tggagacatt tgcccccagc tgtgagcagg gacaggtctg gccaccgggc
      241 ccctggttaa gactctaatg acccgctggt cctgaggaag aggtgctgac gaccaaggag
      301 atcttcccac agacccagca ccagggaaat ggtccggaaa ttgcagcctc agcccccagc
      361 catctgccga cccccccacc ccaggcccta atgggccagg cggcaggggt tgacaggtag
      421 gggagatggg ctctgagact ataaagccag cgggggccca gcagccctca gccctccagg
      481 acaggctgca tcagaagagg ccatcaagca ggtctgttcc aagggccttt gcgtcaggtg
      541 ggctcagggt tccagggtgg ctggacccca ggccccagct ctgcagcagg gaggacgtgg
      601 ctgggctcgt gaagcatgtg ggggtgagcc caggggcccc aaggcagggc acctggcctt
      661 cagcctgcct cagccctgcc tgtctcccag atcactgtcc ttctgccatg gccctgtgga
      721 tgcgcctcct gcccctgctg gcgctgctgg ccctctgggg acctgaccca gccgcagcct
      781 ttgtgaacca acacctgtgc ggctcacacc tggtggaagc tctctaccta gtgtgcgggg
      841 aacgaggctt cttctacaca cccaagaccc gccgggaggc agaggacctg cagggtgagc
      901 caaccgccca ttgctgcccc tggccgcccc cagccacccc ctgctcctgg cgctcccacc
      961 cagcatgggc agaagggggc aggaggctta agggcgaatt c
//
