LOCUS       sequence-89666-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  904 bp insert from human INS gene used as a control for 
            methylation-specific PCR assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K
  TITLE     Development of a quantitative methylation-specific polymerase chain 
            reaction method for monitoring beta cell death in type 1 diabetes.
  JOURNAL   PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub
            2012 Oct 29.
  PUBMED    23144715
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47942. doi"; pages: " 
            10.1371/journal.pone.0047942. Epub 2012 Oct 29"
COMMENT     Sequence Label: pCR2.1-INS-904
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..28
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10..27
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     complement(317..388)
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
ORIGIN
        1 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatcaagc ttggtaccga
       61 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttagc ctcctgcccc
      121 cttctgccca tgctgggtgg gagcgccagg agcagggggt ggctgggggc ggccaggggc
      181 agcaatgggc ggttggctca ccctgcaggt cctctgcctc ccggcgggtc ttgggtgtgt
      241 agaagaagcc tcgttccccg cacactaggt agagagcttc caccaggtgt gagccgcaca
      301 ggtgttggtt cacaaaggct gcggctgggt caggtcccca gagggccagc agcgccagca
      361 ggggcaggag gcgcatccac agggccatgg cagaaggaca gtgatctggg agacaggcag
      421 ggctgaggca ggctgaaggc caggtgccct gccttggggc ccctgggctc acccccacat
      481 gcttcacgag cccagccacg tcctccctgc tgcagagctg gggcctgggg tccagccacc
      541 ctggaaccct gagcccacct gacgcaaagg cccttggaac agacctgctt gatggcctct
      601 tctgatgcag cctgtcctgg agggctgagg gctgctgggc ccccgctggc tttatagtct
      661 cagagcccat ctcccctacc tgtcaacccc tgccgcctgg cccattaggg cctggggtgg
      721 gggggtcggc agatggctgg gggctgaggc tgcaatttcc ggaccatttc cctggtgctg
      781 ggtctgtggg aagatctcct tggtcgtcag cacctcttcc tcaggaccag cgggtcatta
      841 gagtcttaac caggggcccg gtggccagac ctgtccctgc tcacagctgg gggcaaatgt
      901 ctccaggaga gcaaagccct cacctgggcc actttccaca ttagaccagg agagctggag
      961 gctgcagggc ggggctcttt gcgctgctgt cccca
//
