LOCUS       sequence-89668-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  842 bp insert from mouse Ins2 gene used as a control for 
            methylation-specific PCR assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K
  TITLE     Development of a quantitative methylation-specific polymerase chain 
            reaction method for monitoring beta cell death in type 1 diabetes.
  JOURNAL   PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub
            2012 Oct 29.
  PUBMED    23144715
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47942. doi"; pages: " 
            10.1371/journal.pone.0047942. Epub 2012 Oct 29"
COMMENT     Sequence Label: pCR2.1-Ins2-842
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..28
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10..27
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatcaagc ttggtaccga
       61 gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttgag ctcggaccat
      121 taagtgcctt gctgcctgaa ttctgctttc cttctacctc tgagagagag ctggggactc
      181 ggctgagtta agaacccagc tatcaattgg aactgtgaaa cagtccaagg gacaaagata
      241 ctaggtcccc aactgcaact tcctggggaa tgatgtggaa aaatgctcag ccaaggacaa
      301 agaaagcatc acccactctg gaacaatgtc ccctgctgtg aactggttca tcaggccatc
      361 agggcccctt gttaagactc taattaccct aggactaagt agaggtgttg acgtccaatg
      421 agcgctttct gcagacctag caccagggaa gtgtttggaa actgcagctt cagcccctct
      481 ggccatctgc tgacctaccc cacctggagc ccttaatggg tcaaacagca aagtccaggg
      541 ggcagagagg aggtgctttg gtctataaag gtagtgggga cccagtaacc accagcccta
      601 agtgatccgc tacaatcaaa aaccatcagc aagcaggaag gtactcttct cagtgggcct
      661 ggctccccag ctaagacctc agggacttga ggtaggatat agcctcctct cttacgtgaa
      721 acttttgcta tcctcaaccc agcctatctt ccaggttatt gtttcaacat ggccctgtgg
      781 atgcgcttcc tgcccctgct ggccctgctc ttcctctggg agtcccaccc cacccaggct
      841 tttgtcaagc agcacctttg tggttcccac ctggtggagg ctctctacct ggtgtgtggg
      901 gagcgtggct tcttctacac acccatgtcc cgccgtgaag tggaggaccc acaagccatg
      961 gaagggcgaa ttctgca
//
