LOCUS       sequence-89671-e       11979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY-compatible lentiviral conditional RNAi delivery vector; 
            SFFV-driven expression of TetR and Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 11979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-X-PURO
FEATURES             Location/Qualifiers
     source          1..11979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..87
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(115..134)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     611..631
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    775..1363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(828..848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1246..1257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1366..1382)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1367..1383)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1473..1706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1731..1749)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1732..1749)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2039..2058)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2271..2290)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2390..2412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2450..2468)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2536..2640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2641..2709,2710..3501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2859..2878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3672..4260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4161..4180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4414..4431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4501..4521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4506..4835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4686..4821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5969..6034
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6164..6184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6609..6743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6646..6665)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6700..6719
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6912..7545
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7592..7717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8203..8436
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8621..8665
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8950..9067
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9131..9255
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9280..9285,9286..9303,9304..9310)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9364..10023
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9430..9449)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10365..10670
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10549..10568
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10711..10835)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        10937..11344
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      11353..11362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11359..11979
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(11432..11451)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccaaaaaaga agagaaaggt cgacggcggt ggtgaaggcc gcggcagcct gctgacctgc
       61 ggcgatgtgg aagaaaaccc gggcccgggg gatccaccgg tcgccaccat ggccatgacc
      121 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
      181 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
      241 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
      301 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
      361 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
      421 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
      481 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
      541 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
      601 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
      661 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaagcggc
      721 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
      781 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      841 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
      901 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
      961 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     1021 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     1081 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     1141 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     1201 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     1261 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     1321 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     1381 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     1441 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     1501 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     1561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1681 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     1741 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     1801 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     1861 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     1921 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     1981 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     2041 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     2101 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     2161 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaactacta ctgggctgct
     2221 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     2281 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     2341 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2401 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     2461 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2521 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2581 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2641 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2701 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2761 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2821 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2881 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2941 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3001 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3061 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3121 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3181 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3241 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3301 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3361 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3421 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3481 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3661 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3721 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3781 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3841 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3901 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3961 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4021 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     4081 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4141 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4201 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4261 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4321 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4381 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4441 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     4501 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4561 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4621 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4681 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4741 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4801 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     4861 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     4921 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     4981 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     5041 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     5101 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     5161 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     5221 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     5281 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     5341 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     5401 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     5461 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     5521 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     5581 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     5641 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     5701 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     5761 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     5821 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     5881 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     5941 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     6001 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     6061 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     6121 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     6181 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     6241 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     6301 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     6361 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     6421 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     6481 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     6541 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     6601 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6661 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6721 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     6781 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc
     6841 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     6901 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     6961 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7021 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7081 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7141 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7201 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7261 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7321 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7561 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7621 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7681 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     7741 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     7801 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     7861 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     7921 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     7981 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8041 caccgcacag caagcgctga tcttcagacc tggaggagga gatagggaca attggagaag
     8101 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8161 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8221 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8281 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8341 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8401 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8461 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8521 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8581 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8641 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8701 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8761 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8821 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8881 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     8941 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9001 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9061 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     9121 cgaattatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     9181 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     9241 tatccagtca tattggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     9301 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     9361 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     9421 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     9481 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     9541 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     9601 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     9661 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     9721 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     9781 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     9841 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     9901 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     9961 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
    10021 taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
    10081 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
    10141 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
    10201 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
    10261 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
    10321 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
    10381 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
    10441 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
    10501 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
    10561 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
    10621 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
    10681 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
    10741 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
    10801 tacgtttctc gttcagcttt cttgtacaaa gtggttgata attcctgcag ccccgataaa
    10861 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
    10921 tggcaagcta gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat
    10981 agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata
    11041 tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc
    11101 ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt
    11161 cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta
    11221 tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct
    11281 ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc
    11341 cgggggggat ccgccaccat ggctagatta gataaaagta aagtgattaa cagcgcatta
    11401 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
    11461 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
    11521 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
    11581 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
    11641 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
    11701 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
    11761 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
    11821 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
    11881 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
    11941 tgcggattag aaaaacaact taaatgtgaa agtgggtcg
//
