LOCUS       sequence-89672-e        2136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses halo tagged PTEN protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Fang X, Fu Y, Long MJ, Haegele JA, Ge EJ, Parvez S, Aye Y
  TITLE     Temporally controlled targeting of 4-hydroxynonenal to specific 
            proteins in living cells.
  JOURNAL   J Am Chem Soc. 2013 Oct 2;135(39):14496-9. doi: 10.1021/ja405400k. 
            Epub 2013 Sep 18.
  PUBMED    24015839
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja405400k";
            journalName: "J Am Chem Soc"; date: "2013-10-2- 2"; volume: "135"; 
            issue: "39"; pages: "14496-9"
COMMENT     Sequence Label: pMIR-His-Halo-Tev-PTEN
FEATURES             Location/Qualifiers
     source          1..2136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..891
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             898..918
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
       61 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      121 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      181 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      241 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
      301 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
      361 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
      421 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
      481 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
      541 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
      601 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
      661 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
      721 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
      781 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
      841 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg ctccggagaa
      901 aacttgtatt tccagggctc agggatgaca gccatcatca aagagatcgt tagcagaaac
      961 aaaaggagat atcaagagga tggattcgac ttagacttga cctatattta tccaaacatt
     1021 attgctatgg gatttcctgc agaaagactt gaaggcgtat acaggaacaa tattgatgat
     1081 gtagtaaggt ttttggattc aaagcataaa aaccattaca agatatacaa tctttgtgct
     1141 gaaagacatt atgacaccgc caaatttaat tgcagagttg cacaatatcc ttttgaagac
     1201 cataacccac cacagctaga acttatcaaa cccttttgtg aagatcttga ccaatggcta
     1261 agtgaagatg acaatcatgt tgcagcaatt cactgtaaag ctggaaaggg acgaactggt
     1321 gtaatgatat gtgcatattt attacatcgg ggcaaatttt taaaggcaca agaggcccta
     1381 gatttctatg gggaagtaag gaccagagac aaaaagggag taactattcc cagtcagagg
     1441 cgctatgtgt attattatag ctacctgtta aagaatcatc tggattatag accagtggca
     1501 ctgttgtttc acaagatgat gtttgaaact attccaatgt tcagtggcgg aacttgcaat
     1561 cctcagtttg tggtctgcca gctaaaggtg aagatatatt cctccaattc aggacccaca
     1621 cgacgggaag acaagttcat gtactttgag ttccctcagc cgttacctgt gtgtggtgat
     1681 atcaaagtag agttcttcca caaacagaac aagatgctaa aaaaggacaa aatgtttcac
     1741 ttttgggtaa atacattctt cataccagga ccagaggaaa cctcagaaaa agtagaaaat
     1801 ggaagtctat gtgatcaaga aatcgatagc atttgcagta tagagcgtgc agataatgac
     1861 aaggaatatc tagtacttac tttaacaaaa aatgatcttg acaaagcaaa taaagacaaa
     1921 gccaaccgat acttttctcc aaattttaag gtgaagctgt acttcacaaa aacagtagag
     1981 gagccgtcaa atccagaggc tagcagttca acttctgtaa caccagatgt tagtgacaat
     2041 gaacctgatc attatagata ttctgacacc actgactctg atccagagaa tgaacctttt
     2101 gatgaagatc agcatacaca aattacaaaa gtctga
//
