LOCUS       sequence-89674-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR based C-terminal SRM tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  TITLE     Sakata Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-SRM (sortase recognition motif)-hphMX4
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..288
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(156..180)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        363..706
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcc ccgggttaat taccgggtct ctaccagaaa
       61 ctggcggcgg tagacgttag ggcgcgccac ttctaaataa gcgaatttct tatgatttat
      121 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      181 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      241 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      301 ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
      361 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
      421 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
      481 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg
      541 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
      601 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
      661 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaaaagcc
      721 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
      781 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
      841 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
      901 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
      961 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttg
//
