LOCUS       sequence-89691-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged ATR N-terminal fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A
  TITLE     Preferential binding of ATR protein to UV-damaged DNA.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.
  PUBMED    12011431
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-05-14"; volume: "99(10)"; pages: 
            "6673-8"
COMMENT     Sequence Label: pcDNA4-ATR-N
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccaggatgg actacaagga cgacgatgac aagggggaac atggcctgga gctggcttcc
      181 atgatccccg ccctgcggga gctgggcagt gccacaccag aggaatataa tacagttgta
      241 cagaagccaa gacaaattct gtgtcaattc attgaccgga tacttacaga tgtaaatgtt
      301 gttgctgtag aacttgtaaa gaaaactgac tctcagccaa cctccgtgat gttgcttgat
      361 ttcatccagc atatcatgaa atcctcccca cttatgtttg taaatgtgag tggaagccat
      421 gaggccaaag gcagttgtat tgaattcagt aattggatca taacgagact tctgcggatt
      481 gcagcaactc cctcctgtca tttgttacac aagaaaatct gtgaagtcat ctgttcatta
      541 ttatttcttt ttaaaagcaa gagtcctgct atttttgggg tcctcacaaa agaattatta
      601 caactttttg aagacttggt ttacctccat agaagaaatg tgatgggtca tgctgtggaa
      661 tggccagtgg tcatgagccg atttttaagt caattagatg aacacatggg atatttacaa
      721 tcagctcctt tgcagttgat gagtatgcaa aatttagaat ttattgaagt cactttatta
      781 atggttctta ctcgtattat tgcaattgtg ttttttagaa ggcaagaact cttactttgg
      841 cagataggtt gtgttctgct agagtatggt agtccaaaaa ttaaatccct agcaattagc
      901 tttttaacag aactttttca gcttggagga ctaccagcac aaccagctag cacttttttc
      961 agctcatttt tggaattatt aaaacacctt gtagaaatgg atactgacca attgaaactc
     1021 tatgaagagc cattatcaaa gctgataaag acac
//
