LOCUS       sequence-89702-e         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for expressing mutated Neuroserpin in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, 
            Kris MG, Huse JT, Brogi E, Massague J
  TITLE     Serpins promote cancer cell survival and vascular co-option in brain
            metastasis.
  JOURNAL   Cell. 2014 Feb 27;156(5):1002-16. doi: 10.1016/j.cell.2014.01.040.
  PUBMED    24581498
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-27- 
            27"; volume: "156"; issue: "5"; pages: "1002-16"
COMMENT     Sequence Label: pBabe-Puro-FLAG-Neuroserpin oloop
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     220..242
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     258..274
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             307..330
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cnnnattttt gtgggntgta tcctcgatgg ccacgnntna ccgagncntc atnnnccngn
       61 ttaagntcaa ggtcttttcn nctggcctgc atggncaccc agaccaggtc ccctacatcg
      121 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
      181 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
      241 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatcc
      301 accatggact acaaagacga tgacgacaag atggctttcc ttggactctt ctctttgctg
      361 gttctgcaaa gtatggctac aggggccact ttccctgagg aagccattgc tgacttgtca
      421 gtgaatatgt ataatcgtct tagagccact ggngaagang aaaat
//
