LOCUS       sequence-89705-e        1422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fungal transformation via ATMT. Transformants to be selected by 
            hygromicin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B
  TITLE     A series of vectors for fungal transformation
  JOURNAL   Fungal Genetics Reports, 1997. Vol. 44, Article 19
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genetics Reports, 1997. Vol. 44, Article 19"
COMMENT     Sequence Label: pFGL821
FEATURES             Location/Qualifiers
     source          1..1422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             370..1395
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
ORIGIN
        1 tctagagtcg agtatattga aggagcactt tttgggcttg gctggagcta gtggaggtca
       61 acaatgaatg cctattttgg tttagtcgtc caggcggtga gcacaaaatt tgtgtcgttt
      121 gacaagatgg ttcatttagg caactggtca gatcagcccc acttgtagca gtagcggcgg
      181 cgctcgaagt gtgactctta ttagcagaca ggaacgagga cattattatc atctgctgct
      241 tggtgcacga taacttggtg cgtttgtcaa gcaaggtaag tggacgaccc ggtcatacct
      301 tcttaagttc gcccttcctc cctttatttc agattcaatc tgacttacct attctaccca
      361 agcatccaaa tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
      421 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
      481 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
      541 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
      601 cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt
      661 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag
      721 gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
      781 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
      841 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
      901 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
      961 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     1021 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     1081 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     1141 ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     1201 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     1261 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     1321 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     1381 agggcaaaga aatagagtag atgccgaccg gaaccagtcg ac
//
