LOCUS       sequence-89707-e        1423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fungal transformation via ATMT. Transformants to be selected by 
            hygromicin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B
  TITLE     A series of vectors for fungal transformation
  JOURNAL   Fungal Genetics Reports, 1997. Vol. 44, Article 19
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genetics Reports, 1997. Vol. 44, Article 19"
COMMENT     Sequence Label: pFGL821-8
FEATURES             Location/Qualifiers
     source          1..1423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             371..1396
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
ORIGIN
        1 ctgcaggtcg agtgatattg aaggagcact ttttgggctt ggctggagct agtggaggtc
       61 aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt
      121 tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg
      181 gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat catctgctgc
      241 ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtggacgacc cggtcatacc
      301 ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc
      361 aagcatccaa atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      421 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      481 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      541 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      601 gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc gttcacaggg
      661 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga
      721 ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
      781 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
      841 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
      901 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
      961 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     1021 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     1081 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     1141 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     1201 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     1261 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     1321 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     1381 gagggcaaag aaatagagta gatgccgacc ggaaccagtc gac
//
