LOCUS       sequence-89711-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fungal transformation via ATMT. Transformants to be selected by 
            bialaphos resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B
  TITLE     A series of vectors for fungal transformation
  JOURNAL   Fungal Genetics Reports, 1997. Vol. 44, Article 19
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genetics Reports, 1997. Vol. 44, Article 19"
COMMENT     Sequence Label: pFGL822-8
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..939
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctgcaggtcg agtgatattg aaggagcact ttttgggctt ggctggagct agtggaggtc
       61 aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt
      121 tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg
      181 gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat catctgctgc
      241 ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc cggtcatacc
      301 ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc
      361 aagcaacgct tcgattagga agtaaccatg agcccagaac gacgcccggc cgacatccgc
      421 cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca
      481 agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc
      541 cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc
      601 gcctacgcgg gtccctggaa ggcacgcaac gcctacgact ggacggccga atcgaccgtg
      661 tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg
      721 aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac
      781 ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc
      841 ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg
      901 gttccgcccc gtccggtcct gcccgtcacc gagatttagg tcgac
//
