LOCUS       sequence-89721-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dicistronic vector with PV IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Burman LG, Mauro VP
  TITLE     Analysis of rRNA processing and translation in mammalian cells using
            a synthetic 18S rRNA expression system.
  JOURNAL   Nucleic Acids Res. 2012 Sep;40(16):8085-98. doi: 10.1093/nar/gks530.
            Epub 2012 Jun 20.
  PUBMED    22718970
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks530"; journalName: "Nucleic Acids Res"; date: 
            "2012-09"; volume: "40"; issue: "16"; pages: "8085-98"
COMMENT     Sequence Label: R5 PV
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..962)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 agcggccggc cgccccgact ctagaattac tgctcgttct tcagcacgcg ctccacgaag
       61 ctcttgatgt acttacccat ttcatctgga gcgtcctcct ggctgaagtg gaggcccttc
      121 accttcacga actcggtgtt agggaacttc ttagctccct cgacaatagc gttggaaaag
      181 aacccagggt cggactcgat gaacatctta ggcagatcgt cgctggcccg aaggtaggcg
      241 ttgtagttgc ggacaatctg gacgacgtcg ggcttgcctc ccttaacgag agggatctcg
      301 cgaggccagg agagggtagg ccgtctaacc tcgcccttct ccttgaatgg ctccaggtag
      361 gcagcgaact cctcaggctc cagtttccgc atgatcttgc ttgggagcat ggtctcgacg
      421 aagaagttat tctcaagcac cattttctcg ccctcttcgc tcttgatcag ggcgatatcc
      481 tcctcgatgt caggccactc gtcccaggac tcgatcacgt ccacgacact ctcagcatgg
      541 acgatggcct tgatcttgtc ttggtgctcg taggagtagt gaaaggccag acaagccccc
      601 cagtcgtggc ccacaaagat gattttcttt ggaaggttca gcagctcgaa ccaagcggtg
      661 aggtacttgt agtgatccag gaggcgatat gagccattcc cgctcttgcc ggacttaccc
      721 attccgatca gatcagggat gatgcatcta gccacgggct cgatgtgagg cacgacgtgc
      781 ctccacaggt agctggaggc agcgttacca tgcagaaaaa tcacggcgtt ctcggcgtgc
      841 ttctcggaat catagtagtt gatgaaggag tccagcacgt tcatttgctt gcagcgagcc
      901 caccactgag gcccagtgat catgcgtttg cgttgctcgg ggtcgtacac cttggaagcc
      961 atggtgatac aattgtctga ttgaaataac tgttgaaatt g
//
