LOCUS       sequence-89722-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 600, Emission: 650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mNeptune2-C1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..770
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    771..836
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc
       61 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
      121 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc ggcagaatca
      181 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc tgcttcatgt
      241 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgatttcttt aagcagtcct
      301 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgcttaccg
      361 ttacccagga caccagcctc caggacggct gcttgatcta caacgtcaag ctcagagggg
      421 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccagta
      481 ccgagaccct gtaccccgct gacggcggcc tggaaggcag atgcgacatg gccctgaagc
      541 tcgtgggcgg gggccacctg cactgcaacc tgaagaccac atacagatcc aagaaacccg
      601 ctaagaacct caagatgccc ggcgtctact ttgtggaccg cagactggaa agaatcaagg
      661 aggccgacaa tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
      721 tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
      841 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      901 a
//
