LOCUS       sequence-89723-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 584, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: JRed-N1
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(117..122,123..833)
                     /codon_start=1
                     /product="red fluorescent protein KillerRed, a 
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /label=KillerRed
                     /note="mammalian codon-optimized"
                     /translation="GSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNV
                     HAVCETGKLPMSWKPICHLIQYGEPFFARYPNGISHFAQECFPEGLSIDRTVRFENDGT
                     MTSHHTYELDGTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAF
                     IGFTTADGGLMMGHFDSKMTFNGSRAIKIPGPHFVTIITKQMRDTSDKRDHVCQREVTY
                     AHSVPRITSAIGSDED"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggac gaggatggtt
      121 cagagggcgg ccccgccctg ttccagagcg acatgacctt caaaatcttc atcgacggcg
      181 aggtgaacgg ccagaagttc accatcgtgg ccgacggcag cagcaagttc ccccacggcg
      241 acttcaacgt gcacgccgtg tgcgagaccg gcaagctgcc catgagctgg aagcccatct
      301 gccacctgat ccagtacggc gagcccttct tcgcccgcta ccccaacggc atcagccact
      361 tcgcccagga gtgcttcccc gagggcctga gcatcgaccg caccgtgcgc ttcgagaacg
      421 acggcaccat gaccagccac cacacctacg agctggacgg cacctgcgtg gtcagccgca
      481 tcaccgtgaa ctgcgacggc ttccagcccg acggccccat catgcgcgac cagctggtgg
      541 acatcctgcc caacgagacc cacatgttcc cccacggccc caacgccgtg cgccagctgg
      601 ccttcatcgg cttcaccacc gccgacggcg gcctgatgat gggccacttc gacagcaaga
      661 tgaccttcaa cggcagccgc gccatcaaga tccccggccc ccacttcgtg accatcatca
      721 ccaagcagat gagggacacc agcgacaagc gcgaccacgt gtgccagcgc gaggtgacct
      781 acgcccacag cgtgccccgc atcaccagcg ccatcggtag cgacgaggat taaagcggcc
      841 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaa
//
