LOCUS       sequence-89726-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548 / 634, Emission: 
            565 / 662.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: PS-mOrange2-C1
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..746
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     646..669
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    747..812
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga
       61 ataatatggc catcattaag gagttcatgc gcttcaaggt gcacatggag ggcactgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggcca cccctacgag ggctttcaga
      181 ccgctaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctctcatcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaactac gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctctc tgcaggacgg cgagttcatc tacaaggtga
      421 agatgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcagga
      541 tgaggctgaa gctgaaggat ggcggccact acacctccga ggtcaagact acctacaagg
      601 ccaagaagtc cgtgctgctg cccggcgcct acatcgtcgg catcaagctg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg ctccgaggcc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
      841 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      901 acctgaaaca taaaatgaat gcaattgttg ttgtta
//
