LOCUS       sequence-89727-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DsRed2-Peroxisomes-4
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..713
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(219..239)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     631..654
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggcctcctcc gagaacgtca
       61 tcaccgagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg
      121 agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg
      181 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
      241 gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
      301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga
      361 cccaggactc ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga
      421 acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctccaccg
      481 agcgcctgta cccccgcgac ggcgtgctga agggcgagac ccacaaggcc ctgaagctga
      541 aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc
      601 agctgcccgg ctactactac gtggacgcca agctggacat cacctcccac aacgaggact
      661 acaccatcgt ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgagatcgt
      721 acaagtccaa gctgtagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      781 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      841 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
//
