LOCUS       sequence-89728-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mDsRed-N1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..782
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     503..522
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggac aacaccgagg
      121 acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga gggctccgtg aacggccact
      181 acttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag accgccaagc
      241 tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
      301 agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac atgaagctgt
      361 ccttccccga gggcttcacc tgggagcgct ccatgaactt cgaggacggc ggcgtggtgg
      421 aggtgcagca ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg aagttcaagg
      481 gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa gactgccggc tgggagccct
      541 ccaccgagaa gctgtacccc caggacggcg tgctgaaggg cgagatctcc cacgccctga
      601 agctgaagga cggcggccac tacacctgcg acttcaagac cgtgtacaag gccaagaagc
      661 ccgtgcagct gcccggcaac cactacgtgg actccaagct ggacatcacc aaccacaacg
      721 aggactacac cgtggtggag cagtacgagc acgccgaggc ccgccactcc ggctcccagt
      781 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
      841 ttaa
//
