LOCUS       sequence-8973-ex        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: Flag pLJM1 Rap2A
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    725..842
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
       61 gcgctaccgg tatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga
      121 cgatgcgcga gtacaaagtg gtggtgctgg gctcgggcgg ggtaggcaaa tccgccctga
      181 ccgtgcagtt cgtgaccggc accttcatcg agaaatacga ccccaccatc gaggacttct
      241 accgcaagga gatcgaggtg gattcgtcgc cgtcggtgct ggagatcctg gacacggcgg
      301 gcaccgagca gttcgcgtcc atgcgggacc tgtacatcaa gaacggccag ggcttcatcc
      361 tcgtctacag cctcgtcaac cagcagagct tccaggacat caagcccatg cgggaccaga
      421 tcatccgcgt gaagcggtat gagaaagtgc cagtcatctt ggttgggaac aaagtggacc
      481 tggaaagtga gagagaagta tcgtccagcg aaggcagagc ccttgctgaa gagtggggct
      541 gcccctttat ggaaacttcc gctaagagta aaacaatggt ggacgaactc tttgcagaaa
      601 ttgtgaggca gatgaactat gctgctcagc ctgacaaaga tgacccatgc tgttctgcat
      661 gtaacataca ataggatatc ttcgaattct cgacctcgag acaaatggca gtattcatcc
      721 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      781 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      841 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
      901 ttgcgccttt tccannnagc cctgggtttg cgcngnncgc ggctgctctg ggcgtgnncc
      961 gggaaacgca ncggcgccga ncctggnctc gcacattcnt cacgtcgtcg cancgtcacc
     1021 c
//
