LOCUS       sequence-89730-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Tiar
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             356..379
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgattgaa ttcaccatgg aaatggacta
      361 caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg aggatgatgg aagacgacgg
      421 acagccccgg actctatacg taggtaacct ctccagagat gtgacagaag tccttattct
      481 tcagttattc agtcagatcg gaccctgtaa aagctgtaaa atgataacag agcatacaag
      541 caatgaccca tattgctttg tggaatttta tgaacacaga gatgcagctg ctgcattagc
      601 tgctatgaat gggagaaaaa ttttgggaaa ggaggtcaaa gtaaactggg caacaacacc
      661 aagtagccag aaaaaagata cttccaatca cttccatgtg tttgttgggg atttgagtcc
      721 agaaattaca acagaagata tcaaatcagc atttgccccc tttggtaaaa tatcggatgc
      781 ccgggtagtt aaagacatgg caactggaaa gtccaaaggc tatggttttg tatcttttta
      841 taacaaactg gatgcagaga atgcaattgt gcatatgggg ggtcagtggt tgggaggtcg
      901 acaaatcaga accaattggg
//
