LOCUS       sequence-89734-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: Kusabira Orange-C1 (KO)
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..684
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     misc_feature    685..750
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtacc atggtgagtg tgattaaacc agagatgaag
       61 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc
      121 acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat ggccaagggc
      181 gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagacct
      241 tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg
      301 tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata
      361 agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc
      421 gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact
      481 gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc
      541 aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct taaaatgcca
      601 ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagctg
      661 gtagaagatg cagtagctca ttcctccgga ctcagatctc gagctcaagc ttcgaattct
      721 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
      781 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      841 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatgg
//
