LOCUS       sequence-89735-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 529, Emission: 539.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZsYellow1-N1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..796
                     /codon_start=1
                     /product="Zoanthus yellow fluorescent protein"
                     /label=ZsYellow1
                     /note="mammalian codon-optimized"
                     /translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
                     LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
                     CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
                     DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
                     AIAFPSALA"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggcccaca gcaagcacgg
      121 cctgaaggag gagatgacca tgaagtacca catggagggc tgcgtgaacg gccacaagtt
      181 cgtgatcacc ggcgagggca tcggctaccc cttcaagggc aagcagacca tcaacctgtg
      241 cgtgatcgag ggcggccccc tgcccttcag cgaggacatc ctgagcgccg gcttcaagta
      301 cggcgaccgg atcttcaccg agtaccccca ggacatcgtg gactacttca agaacagctg
      361 ccccgccggc tacacctggg gccggagctt cctgttcgag gacggcgccg tgtgcatctg
      421 taacgtggac atcaccgtga gcgtgaagga gaactgcatc taccacaaga gcatcttcaa
      481 cggcgtgaac ttccccgccg acggccccgt gatgaagaag atgaccacca actgggaggc
      541 cagctgcgag aagatcatgc ccgtgcctaa gcagggcatc ctgaagggcg acgtgagcat
      601 gtacctgctg ctgaaggacg gcggccggta ccggtgccag ttcgacaccg tgtacaaggc
      661 caagagcgtg cccagcaaga tgcccgagtg gcacttcatc cagcacaagc tgctgcggga
      721 ggaccggagc gacgccaaga accagaagtg gcagctgacc gagcacgcca tcgccttccc
      781 cagcgccctg gcctgaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      841 gaggttttac ttgctttaaa
//
