LOCUS       sequence-89746-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: Kusabira Orange-N1 (KO)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..757
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagtg tgattaaacc
      121 agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga
      181 aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat
      241 ggccaagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg
      301 ccacagacct tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc
      361 tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag
      421 tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa
      481 ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga
      541 gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga
      601 aggaggcggc aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct
      661 taaaatgcca ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat
      721 tactgagctg gtagaagatg cagtagctca ttcctaagcg gccgcgactc tagatcataa
      781 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      841 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      901 atggttacaa ataaagcaat agcatcacaa
//
