LOCUS       sequence-89747-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged ATR middle region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A
  TITLE     Preferential binding of ATR protein to UV-damaged DNA.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.
  PUBMED    12011431
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-05-14"; volume: "99(10)"; pages: 
            "6673-8"
COMMENT     Sequence Label: pcDNA4-ATR-M
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccaggatgg actacaagga cgacgatgac aagctctcac ttccatggat ttattcccat
      181 tctgatgatg gctgtttaaa gttgaccaca tttgccgcta atcttctaac attaagctgt
      241 aggatttcag atagctattc accacaggca caatcacgat gtgtgtttct tctgactctg
      301 tttccaagaa gaatattcct tgagtggaga acagcagttt acaactgggc cctgcagagc
      361 tcccatgaag taatccgggc tagttgtgtt agtggatttt ttatcttatt gcagcagcag
      421 aattcttgta acagagttcc caagattctt atagataaag tcaaagatga ttctgacatt
      481 gtcaagaaag aatttgcttc tatacttggt caacttgtct gtactcttca cggcatgttt
      541 tatctgacaa gttctttaac agaacctttc tctgaacacg gacatgtgga cctcttctgt
      601 aggaacttga aagccacttc tcaacatgaa tgttcatctt ctcaactaaa agcttctgtc
      661 tgcaagccat tccttttcct actgaaaaaa aaaataccta gtccagtaaa acttgctttc
      721 atagataatc tacatcatct ttgtaagcat cttgatttta gagaagatga aacagatgta
      781 aaagcagttc ttggaacttt attaaattta atggaagatc cagacaaaga tgttagagtg
      841 gcttttagtg gaaatatcaa gcacatattg gaatccttgg actctgaaga tggatttata
      901 aaggagcttt ttgtcttaag aatgaaggaa gcatatacac atgcccaaat atcaagaaat
      961 aatgagctga aggatacctt gattcttaca acaggggata ttggaagggc cgcaaaagga
     1021 gatttggtac catttgcact cttacactta ttgcattgtt tgttatccaa gtcagcatct
     1081 gtctctgg
//
