LOCUS       sequence-89751-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mWasabi-C1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
     misc_feature    743..808
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcagttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aaggagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagatgaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagatcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
      781 agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
      841 taccacattt gtagaggttt tacttgcttt aaaaacctcc cacacctccc cctgaacctg
      901 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta t
//
