LOCUS       sequence-89756-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP reporter along with FF4 miRNA binding sites and 28 nt sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Constru...
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(159..178)
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    complement(387..406)
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             complement(575..826)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(617..638)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
       61 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
      121 tttcgaaccc cagagtcccg cttacccggg tttcttagct gcctatacgg cagtgaactt
      181 taattaaaga cttcaagcgg tttaattaaa gacttcaagc ggtttaatta aagacttcaa
      241 gcggtttaat taaagacttc aagcggagca ggaaccgatg tggccagagg tttaattaaa
      301 gacttcaagc ggtttaatta aagacttcaa gcggtttaat taaagacttc aagcggttta
      361 attaaagact tcaagcggtt tcttagctgc ctatacggca gtgaactcgc agaccgatac
      421 caggatcttg ccatcctatg gaactgcctc ggtgagtttt gctttttttt tttaaagcta
      481 gggaaaggcc aaaaagcaaa acctgagaaa acaaaaggtt gttttctcag gaaaagaaaa
      541 acctttacaa ccctactgac gaatcaccgg tttacttgta cagctcgtcc atgccgagag
      601 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      661 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
      721 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
      781 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
      841 cgttgtcgct gatggcgttg tactccagct tg
//
