LOCUS       sequence-89762-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtEos-N1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..784
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagtg cgattaagcc
      121 agacatgaag atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      181 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      241 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      301 ggtattcgcc gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg
      361 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga
      421 cataacaatg gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc
      481 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      541 gtatgtgcgt gatggagtgc tgacgggtga tattaccatg gctttgttgc ttgaaggaaa
      601 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      661 accaggctac cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      721 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
      781 ataagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      841 ctttaaa
//
