LOCUS       sequence-89774-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H2B-C-22
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     polyA_signal    586..707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(623..642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     677..696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(851..872)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctgtaca agtccggact cagatctcga gagtcgtccg gtcgcagcgg ctccggcata
       61 agtagcgcat ctggttcccc agagccagcg aagtctgctc ccgccccgaa aaagggctcc
      121 aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag
      181 gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt
      241 tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca
      301 ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc
      361 cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt
      421 actaaggcca tcaccaagta caccagcgct aagtaaggat ccaccggatc tagataactg
      481 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      541 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
      721 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
      781 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
      841 gagtgttgtt ccagtttgga acaagagtcc actattaa
//
