LOCUS       sequence-89778-e        9932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           501..527
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           591..632
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     regulatory      788..797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(794..796,797..799,800..1510)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(794..796,797..799,800..1510)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(839..860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1100..1119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1447..1468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1600..2174,2175..2177,2178..2186)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1767..1784)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1994..2013
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2187..2786
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2187..2206)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2683..2703
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2901..2918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2907..3018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3114..3235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3367..4074)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     promoter        complement(4085..5296)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4229..4248)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5318..5336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5353..5371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5389..5407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5425..5443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5460..5478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5496..5514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5526..5564
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5561..5585
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     ncRNA           5742..5768
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           5794..5835
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             5964..6671
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6109..6127)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6377..6396
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6705..6983
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(7079..7098)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7144..7199
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7198..7217)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    7560..7593
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7606..7727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7746..7766)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7746..7764)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7785..7801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7785..7801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7798..7820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7809..7825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7833..7839,7840..7857,7858..7863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7878..7899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8016..8033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8187..8775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8267..8286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8946..9737,9738..9806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9569..9588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9807..9911)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcaccc tgcagtacgt
      361 atccggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      421 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      481 ttcggtcgac ttctagagct ctggaggctt gctgaaggct gtatgctgat atggattcat
      541 gtgcacatgg ttttggccac tgactgacca tgtgcatgaa tccatatcag gacacaaggc
      601 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      661 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      721 acaggtgtcg gtaccgcggg cccgggatcc accggcgcgc cctgcaggcg gccgcctcga
      781 gccggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      841 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      901 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      961 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1021 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1081 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1141 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1201 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1261 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1321 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1381 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1441 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1501 gctgtacaag tccggataac cggaggtgca ggctgcctat gcatgttaac aattgcatcg
     1561 ataagcttga tatcgaattc ctgcagcccg ggggatccgc ccctctccct cccccccccc
     1621 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     1681 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     1741 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     1801 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     1861 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     1921 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     1981 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     2041 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     2101 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     2161 aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg cgcctcgcca
     2221 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2281 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2341 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2401 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     2461 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     2521 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtgtcgc
     2581 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     2641 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     2701 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     2761 gcatgacccg caagcccggt gcctagccgg agaccgaaag gagcgcacga ccccatggct
     2821 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2881 ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2941 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3001 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3061 ggggcaggac agcaaggggg aggattggga agacaatagc actagtttcg cgttaagata
     3121 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3181 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3241 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3301 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg
     3361 atctatttac ccggggagca tgtcaaggtc aaaatcgtca agggcatcgg ctgggagcat
     3421 gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt
     3481 ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg
     3541 gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg taggtgtctc
     3601 tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg
     3661 gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa
     3721 cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac
     3781 tttagccccg tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag
     3841 aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtatgatgcc tgtccagcat
     3901 ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg
     3961 ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgacacc
     4021 attgagtaat tccagagctc cgtttatgac tttgctcttg tccagcctag acatggtggc
     4081 tagcgtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac
     4141 actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac
     4201 aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa
     4261 gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga
     4321 ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac
     4381 ggagaaaagc ctaccgcaca cctaccggca ggtggcccca ccctgcatta taagccaaca
     4441 gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc
     4501 caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag
     4561 tgacaaactt cacgtcaggg tccccagatg gtgccccagc ccatctcacc cgaataagag
     4621 ctttcccgca ttagcgaagg cctcaagacc ttgggttctt gccgcccacc atgcccccca
     4681 ccttgtttca acgacctcac agcccgcctc acaagcgtct tccattcaag actcgggaac
     4741 agccgccatt ttgctgcgct ccccccaacc cccagttcag ggcaaccttg ctcgcggacc
     4801 cagactacag cccttggcgg tctctccaca cgcttccgtc ccaccgagcg gcccggcggc
     4861 cacgaaagcc ccggccagcc cagcagcccg ctactcacca agtgacgatc acagcgatcc
     4921 acaaacaaga accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc
     4981 gcgtccttat ataatcatcg gcgttcaccg ccccacggag atccctccgc agaatcgccg
     5041 agaagggact acttttcctc gcctgttccg ctctctggaa agaaaaccag tgccctagag
     5101 tcacccaagt cccgtcctaa aatgtccttc tgctgatact ggggttctaa ggccgagtct
     5161 tatgagcagc gggccgctgt cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc
     5221 ccttcgtctg acgtggcagc gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa
     5281 acccggcgcg gaggccacgc gtactagacg agtttactcc ctatcagtga tagagaacga
     5341 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     5401 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     5461 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     5521 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     5581 agatcgcacc gggtgactct cttaaggtag ccttgcagaa gttggtcgtg aggcactggg
     5641 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     5701 agacagagaa gactcttgcg tttcggtcga cttctagagc tctggaggct tgctgaaggc
     5761 tgtatgctag agacgtacag atgcgtctca caggacacaa ggcctgttac tagcactcac
     5821 atggaacaaa tggccgaatt cggcgctagc tgctgatatc gcatacgcgt ggaccagata
     5881 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tcggtaccgc
     5941 gggcccggga tccaccggtc accatggtga gcaagggcga ggaggataac atggccatca
     6001 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     6061 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     6121 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6181 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     6241 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     6301 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     6361 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     6421 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     6481 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     6541 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     6601 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6661 agctgtacaa gggtaccgcg ggcccgggat ccaccggatc tagaatcctc tggcatgaga
     6721 tgtggcatga aggcctggaa gaggcatctc gtttgtactt tggggaaagg aacgtgaaag
     6781 gcatgtttga ggtgctggag cccttgcatg ctatgatgga acggggcccc cagactctga
     6841 aggaaacatc ctttaatcag gcctatggtc gagatttaat ggaggcccaa gagtggtgca
     6901 ggaagtacat gaaatcaggg aatgtcaagg acctcctcca agcctgggac ctctattatc
     6961 atgtgttccg acgaatctca aagactagat aagtacaagt aaacactcag gtgcaggctg
     7021 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     7081 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     7141 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     7201 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     7261 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     7321 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     7381 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     7441 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     7501 gtccctcttc tcttatgaag atccctcgac ctgagcgtcg agctagcata tggatccata
     7561 taacttcgta taatgtatgc tatacgaagt tattacgata ccgtcaactt gtttattgca
     7621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt acccagcttt
     7741 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     7801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9901 ggggttccgc gcacatttcc ccgaaaagtg cc
//
