LOCUS       sequence-89797-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag tagged human oncogenic BRAFV600E from bleomycin 
            resistance retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEbleo-Flag-BRAFV600E
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..512
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
       61 ggcctaggct tttgcaaaaa gcttgccagc tggggcgccc tctggtaagg ttgggaagcc
      121 ctgcagatct gatcaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
      181 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
      241 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
      301 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
      361 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
      421 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
      481 gcgtgcactt cgtggccgag gagcaggact gaccgacgcc gaccaacacc gccggtccga
      541 cgcggcccga cgggtccgag gcatcgataa aataaaagat tttatttagt ctccagaaaa
      601 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
      661 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
      721 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      781 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      841 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      901 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      961 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     1021 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     1081 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     1141 gctgttcctt g
//
