LOCUS       sequence-89802-e         370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human MEK1 from bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-MEK1
FEATURES             Location/Qualifiers
     source          1..370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..36
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     181..203
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             292..318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattataca
       61 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
      121 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      181 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagatcg
      241 tcagtcagtc acgatgcggc cgctcgagtc gacgatatcg ccgccaccat gtacccatac
      301 gatgttccag attacgctcc caagaagaag ccgacgcccc agcacaccaa cccatgctgc
      361 tggcgtctaa
//
