LOCUS       sequence-89806-e        3916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAT1Z1A
FEATURES             Location/Qualifiers
     source          1..3916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(10..49)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(53..844,845..913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(914..1005)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      1165..1259
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3405..3793
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(3824..3910)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagt ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg gtttaccaat
       61 gtttaatcag gctcgcgcca atttccgcaa tctgacggtt acgttcatcc atcgtcgcct
      121 ggctgccggt ggtataaatc accacaatac ggctcggttt accatccggg cccagcgccg
      181 caataatgcc acggctgcca cgttcgcccg caccgctttt atccgcaata aaccagccag
      241 ccggcagcgc gctacgcagc agcggacccg ccactttatc cgcttccatc caatcaatca
      301 gttgctggcg gcttgccagg gtcagcagct cacccgtcag cagtttacgc agggtggtcg
      361 ccattgctgc cggcatggtg gtatcacgtt catcgttcgg aatcgcttcg ttcagttccg
      421 gttcccaacg atccagacgg gtcacatgat cgcccatgtt atgcagaaac gcggtcagtt
      481 ctttcggacc gccaatggtc gtcagcagca ggttcgccgc ggtgttatcg ctcatggtaa
      541 tcgccgcgct gcacagttca cgcacggtca tgccatcggt cagatgtttt tcggtcaccg
      601 ggctatattc caccagatcg ttctggctat aatgaatacg acggcccagt tgttcctggc
      661 ccgcatccac acggctcaga accgcaccgc acagcagcac tttaaaggtg ctcatcatcg
      721 gaaaacgttc ttccggacga aaagattcca gaattttgcc gctgttcaga tccagttcaa
      781 tatagcccac gcgcgcaccc agttgatctt ccgcatcttt cactttcacc agggtttccg
      841 gatgcgcaaa caccggcagg caaaacgccg caaaaaacgg aatcagcgcc acacgaaaat
      901 gctgaatgct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gaattaaaaa
     1021 aaagcccgct cattaggcgg gctactacct aggccgcggc cgcgcgaatt cgagctcggt
     1081 acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg
     1141 ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac
     1201 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1261 caggggtccc caataattac gatttaaatc acagcaaaca ccacgtcggc cctatcagct
     1321 gcgtgctttc tatgagtcgt tgctgcataa cttgacaatt aatcatccgg ctcgtagggt
     1381 ttgtggaggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg
     1441 aaacatctta atcatgctgg ggagggtttc taatgaaaca gtatttagaa ctgatgcaaa
     1501 aagtgctcga cgaaggcaca cagaaaaacg accgtaccgg aaccggaacg ctttccattt
     1561 ttggtcatca gatgcgtttt aacctgcaag atggattccc gctggtgaca actaaacgtt
     1621 gccacctgcg ttccatcatc catgaactgc tgtggtttct tcagggcgac actaacattg
     1681 cttatctaca cgaaaacaat gtcaccatct gggacgaatg ggccgatgaa aacggcgacc
     1741 tcgggccagt gtatggtaaa cagtggcgtg cctggccaac gccagatggt cgtcatattg
     1801 accagatcac tacggtactg aaccagctga aaaacgaccc ggattcgcgc cgcattattg
     1861 tttcagcgtg gaacgtaggc gaactggata aaatggcgct ggcaccgtgc catgcattct
     1921 tccagttcta tgtggcagac ggcaaactct cttgccagct ttatcagcgc tcctgtgacg
     1981 tcttcctcgg cctgccgttc aacattgcca gctacgcgtt attggtgcat atgatggcgc
     2041 agcagtgcga tctggaagtg ggtgattttg tctggaccgg tggcgacacg catctgtaca
     2101 gcaaccatat ggatcaaact catctgcaat taagccgcga accgcgtccg ctgccgaagt
     2161 tgattatcaa acgtaaaccc gaatccatct tcgactaccg tttcgaagac tttgagattg
     2221 aaggctacga tccgcatccg ggcattaaag cgccggtggc tatctaagac ttttgtcagg
     2281 ttcctactgt gacgactacc accgatagac tggagtgttg ctgcgaaaaa accccgccga
     2341 agcggggttt tttgcgagaa gtcaccacga ttgtgcttta cacggagtag tcggcagttc
     2401 cttaaatacc tggaagttta ggtgttttgg gtattggtgg tgtaggtggc tgcaaacttt
     2461 ccaatttttg ttgaattgct ttaaattcag gtgtctcttg gtgtttattt agaaccattt
     2521 tttgctctat tcgttctaaa gtcggttgaa tttctttccc tgatacttta cgattcaatt
     2581 ctttttctaa ggtttctttt ggactaatag aaggtgtttc caaacttgaa tagagtttta
     2641 ctccttctct gttatatagc cttatatcgc taaataagcc cgttttatga agggtttcta
     2701 aattggtcgg taagtttttg acaacaatat catgcgcttg ttttggtgtt gccctggctg
     2761 tcattggatc atctgcatac atggtttcat atcgttcaat tgttcctaaa tatgagttga
     2821 ttttaggtac tgccatgaca tacatttttg tttcataacc tttggcttga agcattgttg
     2881 cggtttgaat aggaacgtct gttgttcgtc ctgtaccttc gatcaccaaa ttataccctt
     2941 gatcgctcaa acggcttatg atcgcttctg tcatgcgatt agaataaggg gtaacgtgtt
     3001 ttactacgtc tttttcataa agtttcacaa gttcatcaaa attagggtgc tgttgtttaa
     3061 aggtatcatt atcaatgaca ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca
     3121 aactggtttt ccctgaccct ggttgcccac caagtaaaaa agcggttggc gattcaaccg
     3181 ctttttttcc ttgaatcaat tcttctaaat tatcatttaa gcgattctca aattgtttgt
     3241 cagtaaaatt gactatattt gccatctagt attgcccgag tatctctagt aacatttatt
     3301 gtacaacacg agcccatttt tgtcaaataa attttaaatt atatcaacgt taataagacg
     3361 ttgtcaataa aattattttg acaaaattgg ccggccggcg cgccgatctg aagatcagca
     3421 gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
     3481 taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct
     3541 catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa
     3601 aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa
     3661 agaatatata aggcttttaa agcctttaag gtttaacggt tgtggacaac aagccaggga
     3721 tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac
     3781 ttaacggctg acatggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     3841 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     3901 gttttatttg atgcct
//
