LOCUS       sequence-89810-e        3468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD1K1A
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(10..49)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(53..844,845..913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(914..1005)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      1165..1259
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2957..3345
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(3376..3462)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagt ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg gtttaccaat
       61 gtttaatcag gctcgcgcca atttccgcaa tctgacggtt acgttcatcc atcgtcgcct
      121 ggctgccggt ggtataaatc accacaatac ggctcggttt accatccggg cccagcgccg
      181 caataatgcc acggctgcca cgttcgcccg caccgctttt atccgcaata aaccagccag
      241 ccggcagcgc gctacgcagc agcggacccg ccactttatc cgcttccatc caatcaatca
      301 gttgctggcg gcttgccagg gtcagcagct cacccgtcag cagtttacgc agggtggtcg
      361 ccattgctgc cggcatggtg gtatcacgtt catcgttcgg aatcgcttcg ttcagttccg
      421 gttcccaacg atccagacgg gtcacatgat cgcccatgtt atgcagaaac gcggtcagtt
      481 ctttcggacc gccaatggtc gtcagcagca ggttcgccgc ggtgttatcg ctcatggtaa
      541 tcgccgcgct gcacagttca cgcacggtca tgccatcggt cagatgtttt tcggtcaccg
      601 ggctatattc caccagatcg ttctggctat aatgaatacg acggcccagt tgttcctggc
      661 ccgcatccac acggctcaga accgcaccgc acagcagcac tttaaaggtg ctcatcatcg
      721 gaaaacgttc ttccggacga aaagattcca gaattttgcc gctgttcaga tccagttcaa
      781 tatagcccac gcgcgcaccc agttgatctt ccgcatcttt cactttcacc agggtttccg
      841 gatgcgcaaa caccggcagg caaaacgccg caaaaaacgg aatcagcgcc acacgaaaat
      901 gctgaatgct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gaattaaaaa
     1021 aaagcccgct cattaggcgg gctactacct aggccgcggc cgcgcgaatt cgagctcggt
     1081 acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg
     1141 ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac
     1201 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1261 caggggtccc caataattac gatttaaatc acagcaaaca ccacgtcggc cctatcagct
     1321 gcgtgctttc tatgagtcgt tgctgcataa cttgacaatt aacatccggc tcgtagggtt
     1381 tgtggagggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga
     1441 aacatcttaa tcatgctggg gagggtttct aatgttcacg ggaagtattg tcgcgattgt
     1501 tactccgatg gatgaaaaag gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta
     1561 tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc accactggcg agtccgctac
     1621 cttaaatcat gacgaacatg ctgatgtggt gatgatgacg ctggatctgg ctgatgggcg
     1681 cattccggta attgccggga ccggcgctaa cgctactgcg gaagccatta gcctgacgca
     1741 gcgcttcaat gacagtggta tcgtcggctg cctgacggta accccttact acaatcgtcc
     1801 gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct gagcatactg acctgccgca
     1861 aattctgtat aatgtgccgt cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg
     1921 tctggcgaaa gtaaaaaata ttatcggaat caaagaggca acagggaact taacgcgtgt
     1981 aaaccagatc aaagagctgg tttcagatga ttttgttctg ctgagcggcg atgatgcgag
     2041 cgcgctggac ttcatgcaat tgggcggtca tggggttatt tccgttacgg ctaacgtcgc
     2101 agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg
     2161 cgttattaat cagcgtctga tgccattaca caacaaacta tttgtcgaac ccaatccaat
     2221 cccggtgaaa tgggcatgta aggaactggg tcttgtggcg accgatacgc tgcgcctgcc
     2281 aatgacacca atcaccgaca gtggccgtga gacggtcaga gcggcgctta aacatgccgg
     2341 tttgctgtaa gacttttgtc aggttcctac tgtgacgact accaccgata gactggagtg
     2401 ttgctgcgaa aaaaccccgc cgaagcgggg ttttttgcga gaagtcacca cgattgtgct
     2461 ttacacggag tagtcggcag ttccttaagt cagaatagtg gacaggcggc caagaacttc
     2521 gttcatgata gtctccggaa cccgttcgag tcgttttccg ccccgtgctt tcatatcaat
     2581 tgtccggggt tgatcgcaac gtacaacacc tgtggtacgt atgccaacac catccaacga
     2641 caccgcaaag ccggcagtgc gggcaaaatt gcctccgctg gttacgggca caacaacagg
     2701 caggcgggtc acgcgattaa aggccgccgg tgtgacaatc agcaccggcc gcgttccctg
     2761 ctgctcatga cctgcggtag gatcaagcga gacaagccag atttcccctc tttccatcta
     2821 gtataactat tgtttctcta gtaacattta ttgtacaaca cgagcccatt tttgtcaaat
     2881 aaattttaaa ttatatcaac gttaataaga cgttgtcaat aaaattattt tgacaaaatt
     2941 ggccggccgg cgcgccgatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     3001 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     3061 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     3121 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     3181 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagccttta
     3241 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc
     3301 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatgggg cgcgcccagc
     3361 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3421 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcct
//
