LOCUS       sequence-89823-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged ATR C-terminal fragment kinase 
            dead mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A
  TITLE     Preferential binding of ATR protein to UV-damaged DNA.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.
  PUBMED    12011431
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-05-14"; volume: "99(10)"; pages: 
            "6673-8"
COMMENT     Sequence Label: pcDNA4-ATR-C-KD
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccaggatgg actacaagga cgacgatgac aaggagctaa caagagctta ccttgcgtat
      181 gctgataata gccgagctca agattcagct gcctatgcca ttcaggagtt gctttctatt
      241 tatgactgta gagagatgga gaccaacggc ccaggtcacc aattgtggag gagatttcct
      301 gagcatgttc gggaaatact agaacctcat ctaaatacca gatacaagag ttctcagaag
      361 tcaaccgatt ggtctggagt aaagaagcca atttacttaa gtaaattggg tagtaacttt
      421 gcagaatggt cagcatcttg ggcaggttat cttattacaa aggttcgaca tgatcttgcc
      481 agtaaaattt tcacctgctg tagcattatg atgaagcatg atttcaaagt gaccatctat
      541 cttcttccac atattctggt gtatgtctta ctgggttgta atcaagaaga tcagcaggag
      601 gtttatgcag aaattatggc agttctaaag catgacgatc agcataccat aaatacccaa
      661 gacattgcat ctgatctgtg tcaactcagt acacagactg tgttctccat gcttgaccat
      721 ctcacacagt gggcaaggca caaatttcag gcactgaaag ctgagaaatg tccacacagc
      781 aaatcaaaca gaaataaggt agactcaatg gtatctactg tggattatga agactatcag
      841 agtgtaaccc attttctaga cctcataccc caggatactc tggcagtagc ttcctttcgc
      901 tccaaagcat acacacgagc tgtaatgcac tttgaatcat ttattacaga aaagaagcaa
      961 aatattcagg aacatcttgg atttttacag aaattgtatg ctgctatgca tgaacctgat
     1021 ggagtggccg gagtcagtgc aattagaaag gcagaaccat ctctaaaaga acagatcctt
     1081 g
//
