LOCUS       sequence-89824-e        4617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector: contains constitutive mRFP1, compatible as reporter 
            for E. coli/B. subtilis. See pSEVA3b61 for more info..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA3b67Rb
FEATURES             Location/Qualifiers
     source          1..4617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5..682)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    824..880
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      923..1017
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     oriT            2027..2135
                     /note="incP origin of transfer"
     CDS             complement(2187..3191)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     primer_bind     complement(3712..3729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3883..4471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3963..3982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4525..4611)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagc accggtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt
       61 agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct
      121 gaaccggttt tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt
      181 tcagtttcag acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt
      241 cggtggaagc ttcccaaccc atggtttttt tctgcataac cggaccgtcg gacgggaagt
      301 tggtaccacg cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg
      361 taacggtaac aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg
      421 ggaaggacag tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac
      481 cgtactggaa ctgcggggac aggatgtccc aagcgaacgg cagcggacca cctttggtaa
      541 ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt
      601 cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga
      661 taacgtcttc ggaggaagcc atctagtatt caccaccttt ctctagtaac atttattgta
      721 caacacgagc ccatttttgt caaataaaat ttaaattata tcaacgttaa taagacgttg
      781 tcaataaaat tattttgaca aaattcctag gccgcggccg cgcgaattcg agctcggtac
      841 ccggggatcc tctagagtcg acctgcaggc atgcaagctt gcggccgcgt cgtgactggg
      901 aaaaccctgg cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc
      961 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     1021 ggggtcccca ataattacga tttaaatcct tcaaacttcc caaaggcgag ccctagtgac
     1081 attagaaaac cgactgtaaa aagtacagtc ggcattatct catattataa aagccagtca
     1141 ttaggcctat ctgacaattc ctgaatagag ttcataaaca atcctgcatg ataaccatca
     1201 caaacagaat gatgtacctg taaagatagc ggtaaatata ttgaattacc tttattaatg
     1261 aattttcctg ctgtaataat gggtagaagg taattactat tattattgat atttaagtta
     1321 aacccagtaa atgaagtcca tggaataata gaaagagaaa aagcattttc aggtataggt
     1381 gttttgggaa acaatttccc cgaaccatta tatttctcta catcagaaag gtataaatca
     1441 taaaactctt tgaagtcatt ctttacagga gtccaaatac cagagaatgt tttagataca
     1501 ccatcaaaaa ttgtataaag tggctctaac ttatcccaat aacctaactc tccgtcgcta
     1561 ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc ttgtcactaa gaaaataaat
     1621 gcagggtaaa atttatatcc ttcttgtttt atgtttcggt ataaaacact aatatcaatt
     1681 tctgtggtta tactaaaagt cgtttgttgg ttcaaataat gattaaatat ctcttttctc
     1741 ttccaattgt ctaaatcaat tttattaaag ttcatttgat atgcctccta aatttttatc
     1801 taaagtgaat ttaggaggct tacttgtctg ctttcttcat tagaatcaat ccttttttaa
     1861 aagtcaatcc cgtttgttga agacttttgt ccttttccgc tgcataaccc tgcttcgggg
     1921 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     1981 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     2041 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     2101 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggcctagaa tcaatccttt
     2161 tttaaaagtc aatcccgttt gttgaactac tctttaataa aataattttt ccgttcccaa
     2221 ttccacattg caataataga aaatccatct tcatcggctt tttcgtcatc atctgtatga
     2281 atcaaatcgc cttcttctgt gtcatcaagg tttaattttt tatgtatttc ttttaacaaa
     2341 ccaccatagg agattaacct tttacggtgt aaaccttcct ccaaatcaga caaacgtttc
     2401 aaattctttt cttcatcatc ggtcataaaa tccgtatcct ttacaggata ttttgcagtt
     2461 tcgtcaattg ccgattgtat atccgattta tatttatttt tcggtcgaat catttgaact
     2521 tttacatttg gatcatagtc taatttcatt gcctttttcc aaaattgaat ccattgtttt
     2581 tgattcacgt agttttctgt attcttaaaa taagttggtt ccacacatac caatacatgc
     2641 atgtgctgat tataagaatt atctttatta tttattgtca cttccgttgc acgcataaaa
     2701 ccaacaagat ttttattaat ttttttatat tgcatcattc ggcgaaatcc ttgagccata
     2761 tctgacaaac tcttatttaa ttcttcgcca tcataaacat ttttaactgt taatgtgaga
     2821 aacaaccaac gaactgttgg cttttgttta ataacttcag caacaacctt ttgtgactga
     2881 atgccatgtt tcattgctct cctccagttg cacattggac aaagcctgga tttacaaaac
     2941 cacactcgat acaactttct ttcgcctgtt tcacgatttt gtttatactc taatatttca
     3001 gcacaatctt ttactctttc agccttttta aattcaagaa tatgcagaag ttcaaagtaa
     3061 tcaacattag cgattttctt ttctctccat ggtctcactt ttccactttt tgtcttgtcc
     3121 actaaaaccc ttgatttttc atctgaataa atgctactat taggacacat aatattaaaa
     3181 gaaaccccca tctatttagt tatttgtttg gtcacttata actttaacag atggggtttt
     3241 tctgtgcaac caattttaag ggttttcaat actttaaaac acatacatac caacacttca
     3301 acgcaccttt cagcaactaa aataaaaatg acgttatttc tatatgtatc aagaatagaa
     3361 agaactcgtt tttcgctacg ctcaaaacgc aaaaaaagca ctcattcgag tgctttttct
     3421 tatcgctcca aatcatgcga ttttttcctc tttgcttttc tttgctcacg aagttctcga
     3481 tcacgctgca aaacatcttg aagcgaaaaa gtattcttct tttcttccga tcgctcatgc
     3541 tgacgcacga aaagccctct aggcgcatag gaacaactcc taaatgcatg tgaggggttt
     3601 tctcgtccat gtgaacagtc gcatacgcaa tattttgttt cccatactgc attaatgaat
     3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacgggggc
     4501 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4561 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
