LOCUS       sequence-8983-ex        7155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(30..52)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(246..265)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     365..387
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(425..443)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        511..615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(616..684,685..1476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(834..853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1647..2235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2136..2155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2384
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2381..2397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2381..2397
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2417..2437
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4941..4965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6355..6379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(7148..7155,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      241 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      301 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      361 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
      421 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      481 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     2281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2341 gcctttgagt gagctgatac cagcggataa caatttcaca caggaaacag ctatgaccat
     2401 gattacgcca agctatcaac tttgtataga aaagttgagt gattatagtc tctgttttcg
     2461 ttaattttga attttgcttg ataaggctgc aacaaagatc aggttgacat atattttcag
     2521 taatttattt taacctgtac tctatcactg ccggctataa taagttcttg aataaaataa
     2581 ttttcccgac aaaacatgag tatttctttc gaaaataaaa gtgcaggcta attagagatt
     2641 attctgtaat taactgcata atttgtcacg tgccatagtt ttacattcca ctacgtcata
     2701 gttcttaaaa tactaatctc ctgaaaatag aagtaggtga agaaagttta attatcagtt
     2761 ctaaaatgac aattgatctt tggaatatgt tctgaaacta ccgatcattg aacagatgct
     2821 atttgaatga tatagaattg tatatttgca atttctgaaa cgcgttctta aaggcacaca
     2881 gattaattca aaagggtctg gccgcaaaaa ggtttatggt ggccgatttt gagttttgtg
     2941 tgtgattgct ttttcacaat cagtgttttc aggattatgt gatgaactag atcttcaagt
     3001 ttcgttacat ttcatatgtt ttcggaactc acgaagtaca tattgggtat tgtgctcaaa
     3061 aaattcagca atcagcttcg ctccgctgac tttagaaccc aaaaaaatag tatggccaaa
     3121 ctgactgtgt tacgatcatt tcaatttttc aatacatatt taagatttct aagagtaaga
     3181 aggtcaaaaa ctgttctgga atacatatat atttttcagg ttacaattag tcaaaaagtg
     3241 cactgaaata tacgttttaa tttcacgaat aacccaatta gttcaatgta tttttggtca
     3301 accaacgtta aagtttggct tccaaccaat tatcatttct gatcaaccac aatgtttttt
     3361 ctttatctgc aagttaattt tttattttta tccagatgtt tggcatattt ttcaattctt
     3421 cactagcgcc cacttcttgc acttccggcg ccctgaatct aatgcatctg ttgcaagaat
     3481 tgaaagacca atcaacacat tgttttcttc acgagatact gaagaaaatg aataaaaaca
     3541 gagaaaaaga gccatgtgat tagtgacaac tgttgctaac agataccata gcttggactt
     3601 ggtacgtgat ggcaacgtat gggtcaacaa aaatgattgc agagggggtg caaaacagtc
     3661 aagtcgagaa aatatgaaaa acagaaaaca aagaacagaa aaatgggttt gagagtcagt
     3721 ataatttata aaagaaaaat tgtacataga aattaaccat ttttgtagaa gaagttattt
     3781 ttcaagcatc gttaaaaatt attcaaagca ccttatttca tatttaattt taaacatggt
     3841 taaatgaaca acacggtgcg caatcaggaa aacttgaaat ctgaaactgt tgttgtgatc
     3901 ttcttcgcaa ctgttcagat agcactagtg taatgttaag agtgcgcgaa tataatggaa
     3961 tataatggat cacacctcct gccatcaggt aaacgtctct gttatcacat atttccaact
     4021 attaaatttt taccttttac agttttacat ttttttgaaa aaagtaactt tttgtcttca
     4081 aaatccctga cgaaaatatc aaatatttta atcgagactg cagaggaacc gattgatgat
     4141 ttggaaaatc cagctttacc tgtgtaagaa ctgaaaagtt tcataaccct agggtattcc
     4201 cagttacatt ccccactggc taacaatagc acccagtttt tcatcacctt tcttcaaatt
     4261 tctcggcgat ttgttaaaaa caaaatttgt gtcccttctc tgatatctct atgtctctaa
     4321 acacaagttc atcggaaaac gaaggagggt aggtgttggt tgggctcccg aagtgaaaat
     4381 agaagagcaa gaatagaata ttagagagag agtgcagaga gggcgggata gctcccggga
     4441 ttccgttttc ttcttcttta tcttcaacga tgatgtgtgt gcgtgttgta tagattctgt
     4501 tgctccccca caactcgctc cgaaggctca atacaattca attgatattg gaggagagcc
     4561 taccggagtg ggaggataag aagaaacata agaagaagaa gaagaagaag catgcttctg
     4621 gtttttgatg ctatgaaaac ggcacaaaaa gatgattgag gtcccttttc aataccttct
     4681 ctcatctttc aaatcccatt gaaacctaaa acttctcacc acgctttacc attgttctcc
     4741 aaaaacttat agcaatgtct ataacttttt tatctctgaa aagcagtgtt ccatttttct
     4801 ttttcctatt ttatttcaat tgtttctcac atttcgtttg gattctttgc ttgtcaacca
     4861 gcttcttctt ccacttttac cgtctaattt tcagggcagg gagccatcaa acccacgacc
     4921 actagatcca tctagaaatg acaagtttgt acaaaaaagc aggcttaatg gcgattgttg
     4981 cgcaaggagt ttctacgatt ttgacgacgt ttcagaaaac gttcaaagga ttactcccat
     5041 tgataattct tgtggcttac actctattgg gagcatggat cttctggatg atcgaaggag
     5101 aaaacgaacg agagatgctc attgagcaac aaaaagaaag ggatgaactc atccggcgaa
     5161 ctgtctataa aattaatcaa ttgcaaatca aaagacagag aagattaatg acggccgagg
     5221 aagaatacaa cagaactgca aaagtgttga ctacctttca agaaactttg ggaattgtac
     5281 cagctgatat ggacaaggat attcattgga cattcttggg atcaatcttc tattgcatga
     5341 cagtctatac aactattggt tacggaaaca ttgttcctgg aactggatgg ggtcgttttg
     5401 ctactatcct ttacgcattc attggaattc cactcacagt gctcagtctt tactgtcttg
     5461 gtagtctttt tgccaaggga tgcaaaatgt tgtggagatt ttttttgaag tccaccaggg
     5521 ttgtgagcaa agatttgtca aataagattt ctgaagctgc cgataatatt gaagaaggaa
     5581 caacggcaat tactccatca gccgagaaaa cagaaaataa cgatgatgac ttgttgtctt
     5641 tccccatctc cggcctcttg cttattaccg tcatctgggt cattttctgt gcggtcttgt
     5701 tcaccttcct agaagaatgg gactttggaa catctctcta cttcacattg atttctttca
     5761 caaccatcgg ttttggagac attcttccat cggattacga tttcatgcca attgttggag
     5821 ttcttcttct cattggtctt tctttggtct ctacagtgat gacactcatt caacagcaaa
     5881 ttgaagcatt agcatcggga atgaaagata acattgacca agaatatgct cgtgcattga
     5941 acgaggctcg tgaagacgga gaggttgacg agcacgtaga tccagaagaa gatccggaga
     6001 acaataaaaa gtcctttgac gctgttatct ccagaatgaa ctggtccaag agagggttgt
     6061 attatcttct tcctgatagc cagaagaaag aactcgcaaa gcagtctgag aagaaaatgg
     6121 gaagaaaaag tattaaaatt cagactgata acgatttgtt ggaaacactc attcgggagg
     6181 aaattttgaa ggcggagctc aacaatgaga tgcacaaata tacagcacca cgatcttcac
     6241 accagccaaa acttgtctat tctgatgttc gcgagaagga ggttccaatt gaagttgtcc
     6301 gtgttgagca tttcaatcac ggaaatgaag actatctaga gcatgacatc tagtacccag
     6361 ctttcttgta caaagtggtg atatctgagc tccgcatcgg ccgctgtcat cagatcgcca
     6421 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     6481 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     6541 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     6601 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     6661 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     6721 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     6781 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     6841 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     6901 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     6961 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     7021 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     7081 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     7141 ctgtttccaa cttta
//
