LOCUS       sequence-89837-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-N1
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    26..106
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(129..332)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(158..178)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        333..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
       61 cagaattcga agcttgagct cgagatctga gtccggtagc gctagcggat ctgacggttc
      121 actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      181 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      241 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
      301 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
      361 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
      421 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
      481 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
      541 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
      601 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
      661 actaatgacc ccgtaattga ttactattaa taactaatgc atggcggtaa tacggttatc
      721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      841 tcacaaaa
//
