LOCUS       sequence-89841-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mDsRed-C1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..713
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     437..456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    714..779
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggacaacacc gaggacgtca
       61 tcaaggagtt catgcagttc aaggtgcgca tggagggctc cgtgaacggc cactacttcg
      121 agatcgaggg cgagggcgag ggcaagccct acgagggcac ccagaccgcc aagctgcagg
      181 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
      241 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacatgaag ctgtccttcc
      301 ccgagggctt cacctgggag cgctccatga acttcgagga cggcggcgtg gtggaggtgc
      361 agcaggactc ctccctgcag gacggcacct tcatctacaa ggtgaagttc aagggcgtga
      421 acttccccgc cgacggcccc gtaatgcaga agaagactgc cggctgggag ccctccaccg
      481 agaagctgta cccccaggac ggcgtgctga agggcgagat ctcccacgcc ctgaagctga
      541 aggacggcgg ccactacacc tgcgacttca agaccgtgta caaggccaag aagcccgtgc
      601 agctgcccgg caaccactac gtggactcca agctggacat caccaaccac aacgaggact
      661 acaccgtggt ggagcagtac gagcacgccg aggcccgcca ctccggctcc cagtccggac
      721 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
      781 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      841 taaa
//
