LOCUS       sequence-89842-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 580, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKind2-C1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="FusionRed fluorescent protein derivative that 
                     shows reversible photoswitching with visible light and very
                     fast OFF-switching kinetics"
                     /label=rsFusionRed3
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEAHTETMYPADGGLEGACDMAL
                     KLVGGGHLIANLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    740..805
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tcagaatccg ctagcgctac cggtcgccac catggtgagc gagctgatta aggagaacat
       61 gcccatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact tcccagccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc cacaccgaga cgatgtaccc
      481 cgctgacggc ggcctggaag gcgcatgtga catggccctg aagctcgtgg gcgggggcca
      541 cctgatcgcc aaccttgaga ccacatacag atccaagaaa cccgctacga acctcaagat
      601 gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg acgatgagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg ctggtgatgg
      721 aggtaaaggt ggaggaggtt ccggactcag atctcgagct caagcttcga attctgcagt
      781 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
      841 cacatttgta gaggttttac ttgctttaaa
//
