LOCUS       sequence-89843-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagRFPet-C1
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..785
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    786..851
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtgt
       61 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      121 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      181 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      241 ctaccagctt catgtacggc agcagaacct tcatcaacca cacccagggc atccccgatt
      301 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
      361 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      421 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
      481 gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg
      541 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca
      601 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gaccacagac
      661 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
      721 ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt
      781 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      841 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
      901 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      961 caattgttgt tgttaacttg tttattgcag cttat
//
