LOCUS       sequence-89844-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A
  TITLE     A green-emitting fluorescent protein from Galaxeidae coral and its 
            monomeric version for use in fluorescent labeling.
  JOURNAL   J Biol Chem. 2003 Sep 5. 278(36):34167-71.
  PUBMED    12819206
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 5. 278(36):34167-71."
COMMENT     Sequence Label: mAzami Green-N1 (mAG)
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..785
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             105..785
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcgtgatca
      121 agcccgagat gaagatcaag ctgtgcatga ggggcaccgt gaacggccac aacttcgtga
      181 tcgagggcga gggcaagggc aacccctacg agggcaccca gatcctggac ctgaacgtga
      241 ccgagggcgc ccccctgccc ttcgcctacg acatcctgac caccgtgttc cagtacggca
      301 acagggcctt caccaagtac cccgccgaca tccaggacta cttcaagcag accttccccg
      361 agggctacca ctgggagagg agcatgacct acgaggacca gggcatctgc accgccacca
      421 gcaacatcag catgaggggc gactgcttct tctacgacat caggttcgac ggcaccaact
      481 tcccccccaa cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga
      541 agatgtacgt ggaggacggc gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg
      601 gcggcggcca ctacaggtgc gacttcaaga ccacctacaa ggccaagaag gaggtgaggc
      661 tgcccgacgc ccacaagatc gaccacagga tcgagatcct gaagcacgac aaggactaca
      721 acaaggtgaa gctgtacgag aacgccgtgg ccaggtactc catgctgccc agccaggcca
      781 agtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
      841 gctttaaa
//
