LOCUS       sequence-89852-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-C1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..81
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    205..326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(242..261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     296..315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(333..788)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(470..491)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     682..701
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
       61 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
      121 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      181 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      301 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
      361 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
      421 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      481 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
      541 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
      601 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
      661 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
      721 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
      781 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      901 atattgaa
//
