LOCUS       sequence-89853-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZADH1A-c501
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(82..105)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(124..144)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctc acttatcgtc gtcatccttg taatcatggt ggtgatgatg
      121 gtgcgattgg aagtagaggt tctctgcgat ttcttctgaa atgcaaacta tctgctttcc
      181 aatgttacct cctgtcatca tggactggaa tgcagctccc atgttttcca acccatttat
      241 taccgtctct ttaatcttta gctttccttc tttaaaccac tgactcagct gtagaatgcc
      301 aggctcaaat ttgtctttat aatttaatac cagaaatctt tcccttgtga tgtttctttc
      361 tttctggatt gcctctatag caggggatag cgggggagga taaggcacat ctttgttgta
      421 ctgagaaatt tgaccacaca ggatgatgtg gctgttctca ttcatctgac ttatcactgt
      481 atcactgatg ttaccaccaa cattgtcaaa ataaacatcc actccagctg ggcatgattc
      541 acggagctgt tctgccacat tgtctttttt ataattaatt gcagcatcaa agcccagttc
      601 tgaggtcaag aggatgcatt tctcatgtgt tccacaaatt cccaccactc tggaacaacc
      661 taagaaatgg ccaatctgcc cagccacaga tccacaggca cctgcggccc cactgacaac
      721 cattgtctta ttagatccag cagttatatg acctttttcc tgtatcccaa tcaaggaagt
      781 caaaccaggc atacctatag ctccaagaaa atatgaaagg tgtccatcca caagttgtgg
      841 gtctaccttt tcaaggctat ttccatccag aataaccttg gtttgccagg gccaatagaa
      901 agaagtcaca aaatcgcctt tagtcaaatt tgtgtgtttg ctttc
//
